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This is clearly an upper estimate, and given that the top 609 nucleotide clusters could be collapsed with less conservative clustering criteria into 85 larger clusters (see Materials and Methods), the actual number of transcripts could be 7-fold or more lower.
However, due to the poor knowledge on resistance against this antibiotic and the methodological difficulties in testing susceptibility with disk diffusion methods, the actual level of resistance against colistin is largely unknown [ 20, 21].
It is well known that due to high discordance between assays in different detection methods, the actual discrepancy is difficult to explain in the prevalence of HEV among different studies.
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For the T-shirt size method the actual average value was 92% (SD=4%) and the desired value was 93% (SD=5%).
With the no net flux method, the actual concentration of glucose and urea in subcutaneous tissue was measured.
For the story points method the actual mean value was 48% (Standard Deviation (SD)=36%) and for the desired value it was 64%SD=32%2%).
Using linear and non-linear regressions and conjoint analysis as method, the actual transactional data sets of Dianping.com one of Asia's largest online group-buying businesses is studied.
Figure 21 Results obtained by applying the previously reported methods and the proposed method to the actual LR image shown in Figure 19a: (a) LR image.
Figure 22 Results obtained by applying the previously reported methods and the proposed method to the actual LR image shown in Figure 20a.
Although having advanced plausibility checks, tariff calculation methods incorporate the actual cost situation better in other countries.
The tested numerical example shows the proposed methods extend the actual concept of topology optimization and are efficient to generate reasonable design patterns.
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