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Because of the expertise and time required for the microscopic discrimination of species, molecular methods that monitor environmental concentrations of Pseudo-nitzschia provide a rapid alternative for the early detection of blooms and prediction of toxin accumulation.
High-throughput methods that monitor genome-wide transcriptional changes can help further characterize at the molecular level the pathways regulated by defense-related genes.
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Coulometry is a method that monitors the quantity of electricity (coulombs) that are consumed during an electrochemical reaction involving the analyte.
Factorial design (FD) is a method that monitors the interactions of multiple factors which accommodate the effect of both main and interaction effects (Cheong and Gupta (2005)).
Urease activity and kinetics were assayed by the Worthington method that monitors urea hydrolysis by coupling ammonia production to a glutamate dehydrogenase reaction.
This paper describes a novel micro-array based method that monitors the β chain repertoire with a resolution of a single T-cell clone.
CDC activity was assayed by an AlamrBlue-based method that monitors the amount of viable cells.
Transient repair activities in the absence of distinct foci can be probed by measuring protein mobility using Fluorescence Recovery After Photobleaching (FRAP); a method that monitors the replenishment of fluorescent proteins into a previously photobleached area inside single cells (Fig. 2A).
This method is, however, widely used for research [ 40– 42], as it is practical and cost effective, especially compared to methods that require monitoring drug concentrations.
One can envisage correlative approaches based on advanced LM techniques, such as time-lapse microscopy, or FRET-based methods that allow monitoring of different functional states of molecules in living cells.
However, to maximize the potential of novel therapy, noninvasive imaging methods that can accurately monitor early treatment response would be crucial to promote individualized therapy.
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