Sorted LIN−/PI− cells were cultured briefly in low serum-containing media for ∼1 hour (Figure 1A, Materials and Methods), stimulated for 5 min with Scf (100 ng/ml) or Thpo (50 ng/ml), fixed and permeabilized as above, stained simultaneously for surface and intracellular antigens, and analyzed by flow cytometry (Figure 2A).
Although performed differently, both methods stimulate the proprioceptive system, are easily reproduced and performed, and are low in cost.
Using phytohemagglutinin, interleukin-2, and irradiated peripheral blood mononuclear cells to stimulate CD4+ T cells in a viral outgrowth assay, only 1%% of HIV+ cells were successfully induced, thereby illustrating that current reactivation protocols and methods stimulate only a fraction of the total inducible reservoir [ 30, 31].
Besides gaining traction themselves, these methods have stimulated parallel developments in other areas, such as MCMC algorithms for Bayesian calculations.
A focus group study was designed as this method stimulates debate and explores ideas about the topics introduced.
In contrast to ablative techniques, which aim at increased permeation of the respective antigen, this method stimulates antigen-presenting cells, which showed increased motility and trafficking into draining LNs after irradiation.
This method stimulates thinking passively; from here to thinking actively, there's not a great distance.
Brain imaging methods used in neurolinguistics may be classified into hemodynamic methods, electrophysiological methods, and methods that stimulate the cortex directly.
It's no wonder that Obama's revolutionary fundraising methods are stimulating the saliva glands of politicians everywhere, including Britain.
What they learn could also lead to improved methods for stimulating the uptake of carbon from the atmosphere by soil bacteria to reduce greenhouse gases.
One-of the problems of the Constitution will be to combine guarantees of local elections and other methods of stimulating the people's interest in local government, with the need for a strong executive.
