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a, Sensitivity of various computational prediction methods (see Supplementary Note) for cell-type-specific transcription factor binding sites (TFBSs).

For B. platifrons, nine libraries with insert sizes ranging from 180 bp to 16 kb were constructed using different preparation methods (see Supplementary Table 1a for details) and sequenced by Illumina Hiseq2000 and Hiseq2500 platforms.

Shown is the accuracy of different deconvolution methods when using different reference reconstruction methods (see Supplementary Note 1 for details): DBSCAN with mean-center approach (as devised in previous studies10,11; red), and K-means with mean-centers (purple), median-centers (blue), and harmonic-mean (green).

As automatic parameter estimation performed very poorly on non-exhaustive module detection methods (which include some clustering methods, see Supplementary Note 2), we assigned every unassigned gene to the module with which the average correlation was the highest prior to calculating the cluster validity indices.

Five to 6 days later, dHPC tissue was harvested for immunblot analyses of BDNF (CCK-SAP, n = 8; SAP, n = 7) and DCX (CCK-SAP, n = 8; SAP, n = 8) (see Supplementary Methods; see Supplementary Fig. 3 for uncropped scan of CCK-SAP vs. SAP BDNF blot).

The prepared Ag powder displays its characteristic metallic color suggesting that carbon coating is indeed dependent on precursor choice and such a pyrolysis reaction appears to be thermodynamically more favorable than commercial methods (see Supplementary Fig. S12 and Tables S9 and S10 online).

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The kinetics of the coating, as well as the formed layers stability for washing were evaluated using the SPR method (see Supplementary Note 5).

The same pattern was observable when analyzing the case-control dataset generated with the PM2 phenocopy method (see Supplementary Figure S3).

For further details of the Langendorff heart perfusion method, see Supplementary material online, S1.

To judge whether τ i estimates are problematic, one may develop a sampling based method (see Supplementary Material S2).

Relative quantification to a reference gene (GAPDH) was performed using the ΔΔCt method (see Supplementary material for primer pairs).

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