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Both methods reveal two stable structures, namely one where the molecule is planar, and the other in which the COOCC dihedrals are rotated to ∼ ±80°.
These methods reveal two distinct levels of organization of genetic information encoding.
The mass spectra obtained for the two sample preparation methods reveal two distinct chemical signatures (see Figure 7B).
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Both staining methods revealed two main cell populations: SSEA-4posNanogpos cells and SSEA-4posNanogneg cells (Figure 6).
Analysis of the coding region variants and splice site variants from both enrichment methods revealed two nonsynonymous heterozygous variants in SLC2A10 (c.616G > A, p.Ala206Thr and c.1154C > G, p. Ala385Gly) and a NOTCH1 potential splice site variant (IVS16-4C > T).
Clustering and heatmap analysis of these CSCs (see Methods) revealed two majors species clusters: the Drosophila melanogaster group (D. melanogaster, D. yakuba, D. erecta, D. ananassae) and a cluster that included four outgroup species (D. persimilis, D. pseudoobscura, D. virilis, D. mojavensis) (Additional file 3: Figure S3).
Partitioning the transcriptome fold change dynamics into three clusters (see Methods) revealed three temporal patterns in transcript abundance in response to MazF induction: down-regulation (K1, 460 genes), pulsatile response characterized by an increase in transcript abundance at early times and decrease following a delay (K2, 148 genes) or up-regulation (K3, 331 genes, Fig. 5c).
TEM study of BN films deposited by both PVD and CVD methods revealed three major types of tBN environments in the tBN layer [tBN with its (0002) planes parallel to the growth direction (I), perpendicular to the growth direction (II) and with its (0002) planes forming curvatures in random directions (III)].
All of these methods revealed four well-distinguishable main modules with a large amount of further sub-modules (Figure 4A and Figures S1.10, S1.11 and S1.12 in the Electronic Supplementary Material S1).
Principal component analysis jointly applied to the three information estimates, the underlying response measures (VS max, FR max and CV min), as well as four additional receptive field parameters (characteristic frequency (CF), Q40, threshold and minimum latency, see Materials and Methods) revealed three orthogonal components of temporal processing (Table 1).
O antigen typing using both conventional and molecular methods revealed three O serogroups, namely O139, O130, and O168.
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