Sentence examples for methods restriction from inspiring English sources

The frequencies of variants in exon 1 obtained by PCR SSP were completely comparable to results obtained by previously described PCR methods, restriction fragment length polymorphism (RFLP) and site-directed mutagenesis (SDM).

All the plasmids were constructed using standard cloning methods (restriction and ligation).

Among available GBS methods, restriction site-associated DNA (RAD) has gained popularity for the construction of high-density linkage maps, and several methods with simpler RAD library preparation protocols have been developed.

Amplified fragment length polymorphism (AFLP) is an application of the DNA fingerprinting technique proposed by Vos et al. [ 1], which is a clever combination of two older methods, restriction fragment length polymorphism (RFLP) [ 2] and random amplified polymorphic DNA (RAPD) [ 3- 5], generating a large number of genetic markers from any genomic DNA [ 6].

Amplicons generated in these 27 PCR reactions were pooled and fragmented by two shearing methods: restriction endonuclease AluI (Thermo Fisher Scientific, USA) treatment and mechanical shearing using nebulisers, under several conditions (see below), to obtain a fragment length range between 0.6 and 0.8 kb recommended for pyrosequencing.

In this method restriction endonucleases are displayed in alphabetical order.

Irrespective of the BAC cloning method (restriction enzyme, DNA-shearing; see above) the average BAC insert size has a major impact on the contribution to the physical map.

A novel polymerase chain reaction (PCR) method, restriction site-dependent PCR (RSD-PCR), was designed for rapid new genes cloning from genomic DNA.

Unlike in many other countries, young women in Sri Lanka have the highest rates of suicide amongst women suggesting that method restriction alone may not be adequate in reducing the higher rates in this group.

Moreover, RUT, UBT, and histopathology are unable to discriminate the reinfection or recrudescence, while PCR based method (restriction analysis or sequencing) has capability to indicate either of the two possibilities.

We report here a simplified and effective PCR method, restriction site-dependent PCR (RSD-PCR), which used the natural restriction sites in the genomic DNA to design universal primers, and was applied with a two-round PCR protocol without nested process.