Sentence examples for methods requires two from inspiring English sources

However, vector construction by these methods requires two sequential PCR reactions, and the purification of insert DNA fragments.

Complicated labeling methods requiring two reaction vessels and multiple Sep-Pak purifications can be conducted on it, such as [18F]FDopa and [18F]F-Gln.

All progressive alignment methods require two stages: a first stage in which the relationships between the sequences are represented as a tree, called a guide tree, and a second step in which the MSA is built by adding the sequences sequentially to the growing MSA according to the guide tree.

Both methods require two sequential transformations, the first transformation integrates the URA3 marker into the targeted locus, and the second transformation replaces URA3 with a PCR fragment containing one or a few mutations.

But this method requires two functioning controllers.

The method requires two inputs for processing.

Each iteration using Krotov's method requires two propagations.

The PRISM method requires two parts to compute the scattering of all STEM probes.

The approximate method requires two approximations, which only use simple algebraic functions.

Their proposed method requires two read cycles, one to obtain the centre value and another to obtain the neighbourhood values.

As illustrated in Figure4, the proposed method requires two steps to perform video resampling: the initial image magnification step and the edge enhancement step.