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These methods require skilled personnel and can delay diagnosis and treatment of threatened flap failures.
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This method requires skilled workers (Peterson and Cromwell 1993) and considerable drop between the upstream and downstream water surfaces, which is often not available in flat grade ditches.
Molecular method involving PCR amplification for the detection of V. cholerae [13] has reduced the diagnosis time [14], but PCR method requires skilled professional and expensive infrastructure that is difficult to perform in countries with low resource setting.
Other methods for gastric empting in children have been used such as imaging tests (i.e. magnetic resonance imaging, functional ultrasonography) and imaging procedures, which have had good results; however, the ability to ensure consistency between the studies using this method is less reliable and often require skilled technicians [ 13, 32, 33].
Emerging scientific methods that show small parts of a biological response to gene environment interactions require skilled translation and outreach efforts.
However, conventional methods for the detection of nucleic acids are not ideal for use in the field, as they require skilled operators and complex equipment.
Transmission plants are highly automated operations that require skilled employees.
Multiprocess facilities require skilled engineering analysis and a working knowledge of the available equipment.
Laboratory testing is done by expensive and sophisticated machines called network analyzers that require skilled operators.
Animal vaccination and serum sampling require skilled labour, adding to testing costs.
Although, those methods are very sensitive, they are not adapted for in situ and real time detection of DL as they are time consuming, involve expensive apparatus and require skilled technicians.
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