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The chromatographic methods require high cost solvents in addition to elaborate treatment.
Physical treatment methods require high energy and cause formation of hazardous by-products.
All these methods require high energy and involve the use of toxic chemicals.
These methods require high computational efforts in order to simulate enough scenarios for statistical significance.
Structure determination methods (such as X-ray crystallography and electron cryo-microscopy) can precisely illustrate protein interaction interface, while these structural methods require high concentration of purified proteins.
In methods that use segmentation, a large number of operations per pixel are needed and consequently these methods require high order of computational cost.
Similar(40)
However, those methods require high-density infiltration to obtain monolithic and freestanding inverse structures after template removal.
The available quantitatively reliable methods require higher computational costs than the DFT method [18].
However, the methods require high-DNA input and do not yield information on distinct CpG dinucleotides.
In silico methods require high-performance data storage and computing and have therefore developed only recently.
However, both methods require high-performance liquid chromatography (HPLC) for purification, which limits the possibility of automated synthesis.
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