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Fluorimetric, phosphorimetric, and immunosensor methods require complicated sample pre-treatments.
Laser thermometry methods require complicated optical systems.
However, these 3D analysis methods require complicated setup of a calibrated multi-camera system.
However, these methods require complicated equipment and large quantities of chemicals, resulting in high cost and environmental burdens.
However, all of these methods require complicated hardware, data and image processing software and/or time-consuming data acquisition procedures.
Despite these advantages, PCR methods require complicated procedures and expensive equipment, such as thermocyclers and electrophoresis units, which are not suitable for use in field conditions.
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This method required complicated 3D reconstruction algorithms and could only work for very thin tissues.
However, these methods also require complicated sample preparation or measurement procedures.
The hand-held HSSM mechanical cell lysis method allowed successful, efficient and high-throughput cell lysis within a short period of time compared to conventional methods which require complicated multiple steps and expensive equipment such as a sonicator and centrifuge.
Thus, although these methods do not require complicated or time-consuming procedures, estimation of chromosome III number within cells by real-time PCR (Fukuda et al. 2013a), array comparative genomic hybridization (Abunimer et al. 2016), or whole-genome sequencing would be needed to investigate the underlying events in more detail.
The inkjet printing method does not require complicated additional fabrication process steps such as photoresist mold and metal seed layer formation for electroplating, in order to pattern a 3D metal structure.
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