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The purification of BoNT/B was completed according to the previous methods published by our laboratory without polyethylene glycol (PEG) precipitation and Sephacryl-200 column process [27].
The moisture and lipids content of lees were determined according to the official methods published by the Association of Official Analytical Chemists AOAC Internationall 1997).
These simulations will follow the CASA wetland NEP modeling methods published by Potter et al. [34] for the continental United States coverage.
The physicochemical characteristics of the sample were determined in accordance with the standard methods published by American Public Health Association (APHA 2005).
We classify primary forest into four fragmentation classes defined by pattern: core (intact forest), inner edge (or perforation), outer edge (bordering large non-forest areas) and small forest patches, derived from the methods published by Vogt et al. [51].
Culture maintenance and harvesting of zoospores followed methods published by Rollins-Smith et al. [29].
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HMF concentrations were determined using an HPLC method based on the method published by the International Honey Commission IHCC) [16].
Protein immobilization was carried out according to the method published by Wakayama et al. [18] with minor modification.
Metabolites of PAHs in bile were quantified by use of a modification of the method published by Kammann et al. [35].
Here, we used modified method published by Batús et al. [20] for the synthesis of size- and shape-controlled citrate-stabilized AuNPs.
The method to calculate the CED (v1.08) is based on the method published by ecoinvent version 2.0 and expanded by PRé Consultants for energy resources available in the SimaPro database [12].
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