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This was performed according to the methods previously validated in our laboratory [12], [20], [21].
The SBWC was measured using in-house software and methods previously validated.
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The immunohistochemical staining was analyzed according to a scoring method previously validated.
Both antecedent and concurrent comorbid conditions were defined from Medicare Part A and Part B claims, using a method previously validated for diabetes [ 1].
A secondary outcome measure was duration of hospital stay, measured using a method previously validated by the Pediatric Investigators Collaborative Network on Infections in Canada studies of hospitalized children with RSV infection [ 15].
OTA was determined by an in-house method previously validated for dry pasta (Ng et al. 2009), with extraction and clean-up essentially the same as AOAC Official Method 2000.03 (Entwisle et al. 2000).
This was done because the en face method was previously validated by comparing it with the aortic root Paigen method [4], [7] and because en face assesses the entire aorta and not just 10 transversal sections of the aortic roots.
The novel method was previously validated by determining the solubility of benzoic acid in water at temperatures ranging from 278.5 to 340.9 K and comparing to literature data.
This test method was previously validated in our lab with an accuracy of 92.3% [ 21].
This method was previously validated in spontaneously breathing patients undergoing renal replacement therapy [ 24].
This method was previously validated over the range of 5.00 1,000 ng/mL with 100-μl aliquot volume.
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