The lack of standardization of US examination method previously and settings for PDUS can limit the use of this technique in clinical practice.
This series of ethanol dehydrations effectively preserve the integrity of DNA and RNA in our samples, as we have extensively tested this method previously and obtained fully intact nucleic acids.
To test the accuracy of various methods previously proposed (and one new method) to estimate offence times where the actual time of the event is not known.
Cells were transfected with EGFP-LC3 (#11546; Addgene) [ 16] using methods previously reported and prepared for direct immunofluorescence according to previously published methods [ 7].
All plasmids used in this study were generated using methods previously described and are listed in Supplementary file 2. Yeast strains were grown according to standard procedures.
DNA was extracted from blood samples using a standard phenol/chloroform method and from archival tissue using methods previously described (Wright and Manos, 1990).
We used methods previously described (Thomsen and Caine, 2005, 2007).
Nine donor samples were expanded by outgrowth similar to methods previously described (Blau and Webster, 1981).
Using methods previously described 48 and all births in 2001 2002, two multivariable logistic regression predictive models for pregnancy hypertension were developed.
The InterVA-4 model was applied to the dataset using the methods previously described (14) and as described in detail in the introductory paper of this issue (23).
