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Results indicated that estimates from the various transect methods performed in a similar manner both within beaches and among beaches.
From a literature review including more than fifteen erosion models, we have identified three main methods used to measure these properties: aggregate stability and splash cup detachability, methods performed in the laboratory using only a few grams of soil, and standard plot methods that are based on field plot measurements.
In terms of how these methods performed in reducing the false positive rate, we observed that metabolites with a distribution highly correlated to the underlying population structure, like for example the carotenoids, still retained the highest number of associated markers in all the statistical models.
Sensitivity and reproducibility of the methods performed in our laboratory have been previously reported [ 10].
Figure 1 provides an overview of the methods performed in this study.
The analytical methods used to assess all laboratory variables were the routine methods performed in each hospital.
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Thus, a comprehensive study on how these methods perform in a pathway-based setting is warranted.
Further investigation could be done into the way these methods perform in this more complex situation.
As it unclear how the various methods perform in different situations we evaluate them using a simulation study.
Figure 3 and Figures S1-S2 in Additional file 1 show how well different methods perform in identifying direct interactions in terms of the three reference sets.
We also wondered how well competing methods perform in practice, as opposed to how well they are supposed to perform in theory.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com