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Furthermore, how to accurately quantify the expression of circRNAs is still a challenge since existing methods only count the junction reads, which are easily affected by multiple factors such as sequencing depth, read length, and fragmentation condition during RNA-seq library construction.
These methods only count one sequence per transcript hence will not suffer from transcript length effects.
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This method only counts viable phages that are able to infect and lyse their host.
During this period, more than one infection might have occurred in one individual, whereas our method only counts one.
When quantifying productivity losses, we will use the friction-cost method, which only counts productivity loss for the time it takes to replace a worker.
The boy will only count as an absolute borderline case of 'obese' if no possible method of inquiry could settle whether he is obese.
Several additional comments: The authors state in the methods that "we only counted single nucleotide substations".
Because I only count 17.
But Lewis' figures only count closed positions.
As estimates of divergence based on site-counting and maximum-likelihood methods gave qualitatively equivalent results, only counting estimates are shown here (for a discussion of the different methods, see Bierne and Eyre-Walker 2003).
Our reconstruction method is one such method since our distance estimator only counts the number of shared adjacencies between genomes and the number of linear chromosomes in each of them.
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