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Important information contained in the discrepancies between these evolutions can be extracted and studied with the methods of trees reconciliation.
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A prime reason for this is that reliable methods of tree construction, such as maximum likelihood (ML) and maximum parsimony (MP), require considerable amounts of computational power.
These observations highlight the need to expand the sequence-structure approach to include character-based methods of tree-building (e.g., ML).
Instead, there is a need for improved methods of tree analysis that can be used to discriminate the noise due to outgroups from the phylogenetic signal within the taxon of interest, and to evaluate realistic models of evolution, correcting the retrospective perspective and explicitly recognizing extinction as a driving force.
Differences between the three methods of tree construction were observed.
Authors' response: The methods of tree reconstruction are now described in the materials and methods section.
The different methods of tree construction all led to topologies with three main well supported branches.
Similar results were obtained for all methods of tree reconstruction, reflecting the robustness of PAML in respect to the phylogenetic tree used.
Future examination with other tree statistics or other methods of tree inference may also yield more dramatic differences than we observe here.
With the two probabilistic methods of tree reconstruction, no substantial changes in the topology were observed between the whole and reduced concatenated datasets.
Among the rigorous methods of tree reconstruction that are available, maximum likelihood (ML) and Bayesian inference (BI) have dominated phylogenetic studies in recent years [ 1- 6].
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