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We have developed improved methods of specimen preparation that involve high-pressure freezing followed by freeze-substitution that are particularly well suited for 3-D studies (O'Toole et al., 2003, 2007).
The methods of specimen collection and culture have been described previously [ 3].
In addition, improved methods of specimen preparation for electron microscopy, combined with tomography, provide new opportunities for understanding Golgi structure.
The approach was compatible with standard methods of specimen preparation for epi-fluorescence, including immunofluorescence and did not alter the morphology of fixed tissues.
The use of saliva as a diagnostic specimen avoids the pain, anxiety and infection risk associated with traditional methods of specimen collection, such as blood sampling or tissue biopsy.
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Color changes of composites may be influenced by the differences in resin shades, the chemical activator, initiator, and inhibitor, polymer, type and quantity of filler, oxidation of C=C double bonds, resin thickness, or storage methods of specimens during observation [ 24].
A method of specimen preparation is described to inhibit these effects.
In order to obtain a uniform density throughout the specimen, the compaction method of specimen preparation suggested by Ladd [34] was followed.
In order to obtain a uniform density throughout the specimen, the compaction method of specimen preparation suggested by Ladd [34] was used.
This is not accomplished by the thin foils prepared by the conventional method of specimen preparation, and only cylindrical or conical-shaped specimens with the symmetry axis parallel to the tilting axis would meet this requirement.
FFPE is a standard method of specimen preparation used in the histological analysis of solid tumors.
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