22 In order to compare to other commonly used methods of resting-state analysis, we replicated our data-driven results with a seed-based approach.
Resting SXM and MR-1 cells were prepared based on the method described in "Preparation of resting cells".
Alternatively, PBMC were tested after 96 h of polyclonal stimulation with R-848, IL-2 and β-mercaptoethanol, which is an established method for the activation of resting memory B cells [ 19].
Another method is the assessment of resting energy expenditure using the Harris-Benedict formula [ 15], which is a simple formula that takes into account the patient's weight, height, age, and gender.
Compared to methods based on the observation of resting brain activity, this perturb-and-measure approach (Paus, 2005) readily dissociates functional connectivity (temporal correlations) from effective connectivity (causal interactions), which is defined as the ability of a subset of neurons to causally affect the activity of other groups of neurons (Friston, 2002; Lee et al., 2003).
In our method, we investigated the phagocytosis of resting conidia, whereas Fontaine et al. determined the aggregation behaviour over a longer period of time.
There was no significant difference between the two recording methods with respect to the number of resting phases, duration of individual resting phases and total length of resting phases (Table 4).
Application of our methods to resting-state fMRI data obtained from 22 healthy adults shows that SPC-EN reveals a modular architecture characterized by strong inter-hemispheric links, distinct ventral and dorsal stream pathways, and a major hub in the posterior medial cortex — features that were missed by conventional methods.
But, in the standard method of converting resting ferrous lipoxygenase to a ferric form, by adding one equivalent of lipid hydroperoxide, one unpaired electron is missing in the spin count.
The major differences from our previous methods are the resting of the PBMC after thawing, the use of PVDF instead of MAIP plates, and counting of spots with the AID ELISPOT reader.
