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Although lacking dual radiotracer capability, PET does have advantages over SPECT including established methods of quantitation and superior spatial resolution.
A correlation was found between all three methods of quantitation.
The reported differences in mRNA expression may relate to variable stages of disease, methods of quantitation and species evaluated.
Variables to consider when designing cell proliferation studies include the animal's age, chemical dose and method of treatment, choice and dose of label, time and length that the label is administered, and methods of quantitation.
Exceptions are classically those where high background, poor intensity, or non-contrasting staining occurs; however, these types of limitations also present similar problems with the visual scale, or any other current methods of quantitation.
In addition we compare the +9 (fractional) and decay methods directly Figure 1 figure supplement 2, Figure 5 figure supplement 2. Importantly, both methods of quantitation provide similar numbers as observed in previous comparisons (Nandakumar et al, 2012; Zaug et al, 2013).
Similar(54)
The comparative Ct method of quantitation was used with the Actin gene (DY475300) as a reference.
These findings would not be possible without the non-invasive method of quantitation of bone tumor burden.
Affymetrix ratios are sometimes higher, but that may simply reflect the method of quantitation used in their analysis.
Relative fold change in gene expression was then compared between H1650-ER1 and H1650 or H1650-ER1 spheroids, adherent cells and H1650-ER1 cells using ΔCt method of quantitation.
The specific process used for amplifying circularized asMIPs was tailored to the method of quantitation; for arrays, MIPs were amplified with biotinylated PCR primers before being hybridized to microarrays.
Related(20)
methods of strength
methods of assay
methods of dosage
details of quantitation
methods of analysis
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methods of slaughter
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