Sentence examples for methods of primer from inspiring English sources

Although various methods of primer labeling and SSR allele detection in eucalypts was reported (Ottewell et al. [2005]; Missiaggia and Grattapaglia [2006]; Faria et al. [2011]; Subashini et al. [2013]), difficulties in accuracy of allele sizing continue to exist.

Although there are situations where acceptable levels of limited duplicated text may occur in the methods of Primer Note papers (such as with thermocycler running conditions), any extensive use of published text must be properly referenced so that readers have a clear understanding of any previous dissemination (American Psychological Association, 2010).

As a proof-of-principle demonstration, the method of primer design was applied in colony PCR for identifying plasmid DNA deletion or insertion mutants after site-directed mutagenesis.

Moreover, the method of primer design around the centromeric region was discussed, which would provide insight into fine mapping of the functional genes in plant centromeres.

All transcription start sites are probably not used with the same frequency and it is reasonable to propose that those detected by the not very sensitive method of primer extension are used most often.

The full length of Glu-1 ORFs was obtained by using the method of primer walking and nested deletion.

Full-length sequence was obtained by using a previously described method of primer walking and the 5′/3′ Rapid Amplification of cDNA Ends (RACE) Kit (Roche), which was used to determine the sequence of the segment ends (5 ).

Plasmid DNA of the colony with the ability to cleave 5-bromo-4-chloro-3-indolyl α- l-fucopyranoside (X-Fuc) was isolated and sequenced by the method of primer walking (Geneart).

To illustrate the feasibility and effectiveness of this new method of primer design, we compared it with traditional methods using primers that had already been prelabeled with the fluorescent tag and those used in (1) multiplexed reactions or (2) with each primer pair run individually.

We recognize that the methods section of Primer Notes is particularly vulnerable to self-plagiarism; after all, there are only so many different ways to describe specific steps of a procedure, such as thermocycler running conditions or PCR reactions.

Amplicon sequencing methods consist of primer-walking across the locus/loci of interest, followed by pooling the sometimes large number of PCR reactions prior to sequencing.