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This method could conveniently supplement other methods of mutation detection analysis on large-scale.
The latter may reflect differences in the methods of mutation detection or the intrinsic genetic heterogeneity of HNSCCs.
The families have been comprehensively screened for germline mutation in these genes by conventional methods of mutation analysis and were found negative.
The families selected to this study have been comprehensively screened for germline mutation in these genes by conventional methods of mutation analysis [ 2, 13] and were found negative.
To evaluate the methods of mutation grounding and impact extraction a gold standard corpus was built as an extension to the corpus used by [ 5] containing documents about haloalkane dehalogenases.
Finally, these studies address the issue that such uncommon mutations may have been underestimated due to the methods of mutation screening that have been generally used in the past decade.
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This is the method of "mutation accumulation" (MA).
Lower prevalence of BRAF mutation was reported in studies that used sequencing as the sole method of mutation analysis [ 6].
More recently next generation sequencing (NGS) has led to a hypothesis-null method of mutation identification through complete sequencing of exons at a locus in a single affected individual.
The method of mutation detection was validated by the confirmation of reported K-ras status in CRC cell lines and a good correlation between fresh-frozen and routinely fixed, paraffin-embedded tissue.
One advantage of this method of mutation discovery is that the location of the mismatch-cleaved bands in the gel image can be used to predict within 10 20 bp where mutations fall within the coding regions of the PCR fragment being screened.
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mode of mutation
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methods of instruction
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