Sentence examples for methods of mutagenesis from inspiring English sources

The ECJ's opinion then discusses whether the mutagenesis exemption is frozen in time or can incorporate newer methods of mutagenesis, such as certain gene-editing techniques that do not introduce foreign DNA.

Additional differences could be related to technical differences in two screens including the methods of mutagenesis (insertion duplication vs. transposon insertion) and mutant detection (PCR vs. Southern hybridization).

In contrast to traditional methods of mutagenesis, injection of morpholino oligonucleotides permits much more rapid targeted gene knockdown.

These methods involve introduction of diversity into the antibody genes by various methods of mutagenesis such as error-prone PCR, DNA or chain shuffling, or oligonucleotide-directed PCR, while affinity selection of the variants with decreasing amounts of antigens [ 16].

SCRaMbLE can be used as a novel method of mutagenesis, capable of generating complex genotypes and a variety of phenotypes.

A new biotechnological method of mutagenesis, called activation tagging mutagenesis (ATM), has increased the understanding of development in various plants by intensifying the range of gene expressions and accumulation of secondary metabolites.

Consistent with the EMS method of mutagenesis, these are all point mutations that result in a single amino acid substitution in the ATPalpha protein.

Although in vitro cloning approaches based on the use of high-fidelity polymerases for PCR amplification have significantly improved in recent years, the use of in vivo approaches should allow a more accurate method of mutagenesis due to the use of the cells own high-fidelity replication system which includes proof reading.

To create the His-tagged free-Ig domain of β3 (β3-Ig-6His; Fig. 1 B), nucleotides translating to residues T130-E191 of the mature human protein, and cloned into the plasmid pFastBacMam, were deleted, and the ECD nucleotides were fused to a C-terminal 6His tag using the QuickChange method of mutagenesis (Stratagene, Cambridge, UK).

Selected plasmids derived from both methods of random mutagenesis were further analysed and are listed in Table 2. Site-directed mutagenesis of the virS gene carried on pJIR2056 was performed using a modification of the unique site elimination method [34] with the U.S.E.

Until the very recent advent of these methods of directed mutagenesis, rat mutational strategies were limited to spontaneous mutants (Eker and Mossige, 1961) or random germline mutagenesis by N-ethyl-N-nitrosourea (ENU) (Mashimo et al., 2008) accompanied by target selection (Smits et al., 2006; Zan et al., 2003).