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However, the latter study used slightly different methodology and does not report whether they used paired examinations, which is one of the strengths of our study since a large variation is inherent to the sensitive methods of mitochondrial isolation.
In vivo many methods of mitochondrial transfer between cells have been postulated; however, our observations show that externally presented mitochondria are readily internalized by a wide variety of host cells.
Some methods of mitochondrial isolation preferentially retain certain mitochondrial subpopulations (Krieger et al., 1980), potentially biasing results, especially when extrapolating conclusions to higher levels of organization.
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Here, we demonstrate a method of mitochondrial thermometry (mito-thermometry) based on the thermosensitive characteristics of Rhodamine B methyl ester (RhB-ME).
Here, we develop a method of mitochondrial thermometry based on Rhodamine B methyl ester, which equilibrates as a thermosensitive mixture of nonfluorescent and fluorescent resonance forms.
In this method of mitochondrial replacement therapy, eggs from the mother and donor are fertilized before the mother's nucleus replaces the donor's and the donor's nucleus is discarded.
Other mechanisms are less likely to be involved based on the method of mitochondrial presentation and mitochondrial size.
As an alternative method of mitochondrial depolarization, we used a combination of oligomycin A and antimycin A, inhibitors of the mitochondrial respiratory chain (Supplementary Fig S1A), which similarly induced MIRO, TOM22 and TIMM44 degradation as well as PARP cleavage.
In a number of recent publications, we have stated that the most common or traditional methods of studying mitochondrial functions are outdated and even obsolete, if the goals of the studies are mitochondrial physiology and pathophysiology [ 9– 11].
To confirm growth curves with a second method, independent of mitochondrial function, we used a cytoplasmic membrane-dye (Neuro-DiO, Biotium) whose fluorescence intensity decreases as a consequence of cell divisions.
Located in the sub-Saharan savanna biome of east and southern Africa, the study used methods like analysis of mitochondrial DNA control-region fragments from 122 individuals to learn more about various topics, such as the phylogeography, genetic diversity, demographic history of the species.
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