Sentence examples for methods of methylation from inspiring English sources

Different species and genetic backgrounds or different methods of methylation detection are likely to exhibit different DNA methylation levels and patterns.

These two methods are the most reliable methods of methylation analysis at present [ 10, 15, 16].

These requirements demand well-controlled studies and, ideally, quantitative methods of methylation analysis.

However, low-level mosaicism, as is often the case in pUPD, may not be detected by some methods of methylation analysis [ 21].

Pyrosequencing (PSQ) is preferable to other methods of methylation analysis in that it is processive and quantitative [ 28] and reviewed in [ 29].

One important caveat relates to the importance of validating specific epigenetic changes by using alternative methods of methylation analysis as well as the necessity to link specific epigenetic changes functionally with gene expression.

The 'right' definition of CIMP is still a highly debated topic, as is the ideal gene panel and the appropriate method of methylation detection [12], [24].

To confirm the methylation detected by MSP, an independent method of methylation analysis, COBRA, was used.

The method of methylation analysis (notably, the ability for quantification of methylation) and the particular region(s) of MMR gene promoters evaluated are important factors to take into account in "second hit" analyses.

We have used a robust and reproducible semiquantitative method of methylation analysis, whereas the other studies have either used methylation-specific assays [ 5, 6] or fully quantitative methodologies, which have been performed in an exceedingly small cohorts that are likely to give rise to significant bias [ 7, 8].

In this review, we introduce typical methods, especially those based on developing the method of methylation-loci recognition from the most used, bisulfite conversion, biological recognition, and the newly proposed chemical cleavage.