Newer methods of labeling involving bifunctional chelating agents (which encompass the "3+1" ligand system, Tc(CO 3+1-containing CO 3+1-containingnonichelatesde, water-soluble phydrazinonicotinamideTc-carrying moieties) have added a new dimension for the preparation of novel technetium compounds.
Results and Discussion - Insertion of the fluorophores into LUVs: It is stated that no detectable difference is found comparing two methods of labeling referring to Figure 4.
2) what is the minimum amount of total RNA required to perform a representative amplification? 3) are the direct and indirect methods of labeling the hybridization probe equivalent?
In the lecture portion of my course, the students learn about several different methods of labeling nucleic acids, including nick translation and 3′ end labeling via the "fill-in" technique using the Klenow fragment of E. coli DNA polymerase I.
Moreover, as most current methods of labeling polyA RNA for gene expression analysis show a marked drop off in efficiency with distance from the polyA tail, array features corresponding to 3' UTRs are more likely to show above background signals after hybridization with labeled RNA.
Using dynamic measurement of regional ventilation by the novel method of labeled aerosol deposition, we have demonstrated for the first time that regional ventilation redistributes with increasing tidal volume, suggesting dynamic regional changes in lung mechanics.
Search engines use crawlers, programs that explore the Web by following hypertext links from page to page, recording everything on a page (known as caching), or parts of a page, together with some proprietary method of labeling content in order to build weighted indexes.
Moreover, while additional factors such as spatial resolution and method of labeling must be considered, such first-order dosimetry estimates may be used to aid identification of optimal isotopes for specific imaging purposes.
Firstly, the different object in the image is marked by the method of labeling, and then, the area of each object is calculated to extract the target objects through threshold set.
To avoid working routinely with 50 μg portions of extremely neurotoxic and expensive BoNT-A (LD50 in humans is about 75 ng [6]), developing and establishing the optimal method of labelling BoNT-A with 125I was done with cetuximab.
