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Endocytic, phagocytic, and pinocytic pathways have selectivity to certain ligands and pharmacological or genetic methods of inhibition (to a degree) and deliver cargoes to distinct destinations.
We have shown that blocking the TGF-β pathway using various methods of inhibition - and at various stages of the pathway - results in a significant reduction of invasion and metastasis of breast cancer cells.
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The presence of two binding pockets enabled us to explore a novel method of inhibition by compounds that bridge the adjacent phosphate and glycerol binding sites.
Assays for antifungal activity are usually executed using the method of inhibition crescents, while assays for antimicrobial activity are performed using the deferred plate method or the agar-diffusion method [48, 49].
Using this method of inhibition of the SNARE sorting machinery we recently demonstrated that they play a role in HIV assembly/release.
Finally, in order to avoid overestimation of ATP synthesis flux, the use of this method of inhibition to determine ATP synthesis implies no increase in the activity of mitochondrial ATP-consuming systems consecutively to the inhibitor addition.
By incorporating the mechanistic insights from these recent findings, a combined method of inhibition of roadblocks and application of enhancing factors may yield the most reliable and effective approach in pluripotent reprogramming.
Both observations indicate that the measurements of transition points are unaffected by the groups of genes for which we measured the transition points after either treatment, or the method of inhibition.
The approach is particularly attractive in that it requires minimal assumptions about either the method of inhibition, or the characteristics of the transcript targets (i.e. transcript interference requires the presence of a complementary seed sequence motif and enrichment of this motif is indicative of RNAi activity).
We measured inhibition (Inh; from which we calculated IC50 values as described in Materials and Methods), reversibility of inhibition (Rev) and use-dependence (UD), as well as time constant of onset (τon) and offset (τoff).
Classical methods for inhibition of CME include treatment of cells with hypertonic sucrose or depletion of intracellular potassium (Heuser and Anderson, 1989; Larkin et al., 1983).
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