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Incipiently presenting the motivation and state-of-the-art methods of germ analysis, the challenges and solutions in hardware construction design are pointed out.
In model organisms, two divergent methods of germ cell specification and maintenance are apparent (1– 3).
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In addition, improved diagnostic methods for germ identification and current treatment algorithms were discussed.
To examine outcomes more extensively, we then repeated the transplantation and used the same method of direct comparison of germ cell activity across all transplantations.
Methods for direct genetic modification of germ cells and zygotes have been utilised to generate genetically modified rats without using ES cells (Smits et al., 2006; Geurts et al., 2009; Izsvák et al., 2010).
We first present a novel method for the effective isolation of germ cells and oocytes on the basis of properties of endogenous germ cell development.
We report a simple and efficient method of preferentially editing the genome of germ cells using TALEN, which may enable the generation of F1 frogs with a bi-allelic target-gene mutation through mating of healthy and fertile F0 frogs even when the gene of interest is necessary for viability, normal development, homeostasis or reproduction.
Here we describe an efficient and reliable method for germ-line transformation of L. cuprina using new piggyBac vector and helper plasmids containing the strong promoter from the L. cuprina hsp83 gene to drive expression of the transposase and fluorescent protein marker gene.
The need to develop methods for the production and in vitro cultivation of germ cell lines for genetic manipulation is emphasized [ 89].
For routine identification of Candida isolates, conventional methods including germ tube formation, chlamydospore formation and sugar assimilation tests are universally accepted [3].
We therefore designed a leukapheresis/Treg transfer therapy in which Treg are isolated from leukapheresis products and transfused to patients, and studied large-scale germ-free methods of Treg preparation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com