Methods of euthanasia and reason for selection are consistent with the AVMA Guidelines on Euthanasia.
Thus, we cannot exclude other possible effects of some elements during the study periods, such as anesthetics for MDCT evaluation and methods of euthanasia and necropsy.
For example, pain medications, or different methods of euthanasia, can influence the results of an experiment, making it difficult to compare with previously published findings [ 10, 12, 19].
a For example, monitoring and titration of anesthesia, monitoring and titration of pain control even over-night, using the most humane known methods of euthanasia, avoiding stressed animals, and using the fewest number of animals possible.
AR publications rarely report performing a systematic review to determine the necessity of the research project, rarely report the use of continuous monitoring of the level of anesthesia or pain control, and often do not report the use of acceptable methods of euthanasia [ 7, 11, 12].
While many animal rights groups (including PETA) recommend against the use of lethal traps in the first place, among more mainstream pest-control resources, two methods of euthanasia are usually deemed acceptable for rodents: cervical dislocation and asphyxiation.
