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We tested several methods of dissociating spheroids: trypsinization, calcium chelation with EGTA, mechanical trituration, and allowing spheroids to dissociate spontaneously by plating them in serum-containing media or plating them in spheroid media on plates pre-coated with 2% Matrigel (growth factor reduced, phenol red free; BD Biosciences, Bedford, MA).
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Even reform advocates like Mr. Holt make a point of dissociating themselves from "conspiracy theories".
These sites are also capable of dissociating H2O, resulting in competition between H2O and N2O.
The problem of dissociating translations and rotations, and experimental approaches.
The within-group CA (WCA) has been proposed as an alternative method to dissociate the effects of different amino acid compositions from the effects directly related to synonymous codon usage.
The minimal role of circadian entrainment history on photoperiodic responsiveness was corroborated in Experiment #3, which employed a different method to dissociate circadian and melatonin histories.
By using a new method to dissociate living brain cells and analyze them by fluorescence-activated cell sorting (FACS), we showed that only GalC+ oligodendrocytes had elevated levels of ROS in mutant brains.
"I kind of dissociated.
After the initial tears, and after three days of dissociated shock, the news settled in.
(D ) Top : image of dissociated hippocampal culture plated on MEA.
Commonly employed methods of in vitro testing, including dissociated, organotypic, organ/explant, and 3-D cultures, are reviewed here with specific focus on retaining cell and molecular interactions and physiological parameters that determine cell phenotypes and their corresponding responses to bioactive agents.
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