The SAR value plots show that, irrespective of the method of conjugation, BSA improves the heating efficiency of the system.
This shows that BSA conjugation makes the iron oxide nanoparticles almost non-haemolytic nature, as the haemolytic index is ≤2 %, irrespective of the method of conjugation.
The stability of the BSA-conjugated iron oxide nanoparticles was enhanced when compared to the hydrophilic iron oxide nanoparticles without BSA conjugation, irrespective of the method of conjugation.
It was observed from the results that due to better colloidal stability, the SAR value of the BSA-iron oxide nanoparticles is higher than the iron oxide nanoparticles without BSA, irrespective of the size of the iron oxide nanoparticles and method of conjugation.
Lysine modification has been the most common method of conjugation, but due to its high abundance in proteins there is an increased chance that a modification will occur in the binding region of the agent and render it inactive.
Immunogenicity and functionality of polysaccharide antibodies depends not only on which carrier protein is used, but also on the method of conjugation [ 20].
One of the most popular methods of noncovalent conjugation is to make use of the natural strong binding of (strept avidin for the small molecule biotin.
Recent advances in immunoliposome design include rapid selection of phage antibody-derived scFv for targeting, and methods for conjugation of ligands to existing US Food and Drug Administration-approved liposomal drugs such as pegylated liposomal doxorubicin (Doxil/Caelxy [PLD]).
The development of the method for conjugation of F-AlF with NOTA has allowed F-fluoride to be utilized in chelation labeling for small and large biomolecules.
