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Traditional methods of absorbance ratios or HABA filtrations are of limited utility due to the large UV absorbance cross-section of QDs.
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The union of this technique with the optical methods of UV vis absorbance and FCS allowed for a comprehensive view of the multisite nature of the system.
Meanwhile, some of the spectrophotometric methods recommended the measurement of absorbance in the near UV region where interference most probably occurs [4, 7] or use non specific reagent (Potassium iodide/potassium iodate) that don't offer suitable linearity range [6].
RNA was extracted using the Trizol method and the concentration and ratio of absorbance at 260 nm to 280 nm (A260/A280 ratio) were measured using a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies, Witec, Littau, Switzerland).
The drug loading of the conjugates was determined by UV-spectrophotometery method (Cecil 9000, Cecil Instruments Ltd ,Cambridge, UK) via measurement of absorbance at 383 nm [ 28– 30].
However, the quantity of the standards used should be accurately calculated by gravimetry or other primary methods, as absorbance at a wavelength of 260 nm is not sufficient alone.
Other classical methods of calibration such as those using absolute values of absorbance, initial rates, etc. gave poor results in the cases considered.
MDA content was measured using thiobarbituric acid (TBA) method at absorbance of 532 nm [ 43], and the data was expressed by nmol/mL protein.
The method is based on the consumption of H2O2 by CAT and loss of absorbance at 240 nm.
Graph of absorbance against number of cells was plotted to determine the HDF cells proliferation as per the standard methods.
The change in color seen in glycated products in early studies prompted the use of spectroscopic methods (based on absorbance and fluorescence measurements) for their analysis, but these methods lacked specificity [ 14].
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