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A 5× dry objective (0.15 HC PL Fluotar) and a 20× dry objective (0.7 HC) were used to collect overview images, and a 63× water objective (1.2 PL APO) to collect high resolution images. in vivo FRET was measured by three independent methods; measurement of sensitized YFP emission, measurement of emission spectra, and measurement of the gain in CFP fluorescence upon YFP photo bleaching.
The protection against corrosion was studied by several methods — measurement of potentiodynamic polarization curves, Tafel plot analysis and electrochemical impedance spectroscopy in 3% NaCl solution.
To measure this variable, we developed an approach in which the total AChE-containing homogenate of insect or mammalian samples was exposed to a candidate inhibitor for a defined period of time, after which the unbound inhibitor was removed from AChE by extended dialysis or centrifuge-spin separation through a gel-filtration size-exclusion column (see Methods, "Measurement of AChE inhibition").
Details of surgical methods, measurement of coagulopathic bleeding, and the therapy algorithm have been published.
Insulin secretion was measured in INS1-E cells or mouse islets after exposure to 3 or 16.7 mmol/l glucose (ESM Methods, Measurement of insulin secretion).
Of these methods, measurement of urinary bladder and gastric pressures are the most common clinical applications [ 8, 9, 10, 11, 12].
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Methods: Measurements of NT-proBNP in acute phase in addition to standard assessment of cardiovascular and neurological details.
Supporting method: measurement of the specific surface areas of PG/NF electrodes.
To avoid the cost and discomfort of this method, measurement of sTfR can be used as a reliable index.
By incorporating these data into mathematical models, such as the least squares fit method, measurements of respiratory mechanics can potentially be monitored continuously and without ventilator manipulation.
The methods of measurement of blood pressure were the indirect method following Riva-Rocci: 24-hour blood pressure measurement, and DINAMAP® blood pressure monitor (GE Healthcare Europe GmbH, Freiburg, Germany).
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