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This technique would be very useful as an additional tool to molecular methods largely used in plant biology and microbiology for studying plant-microbe interaction such as RNA-Seq and Proteomics.
Traditional laboratory methods largely used different types of phenotypic assays to perform this important task, although this approach is limited to organisms that can be cultured in a laboratory.
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Such limitations are largely absent in RNA sequencing (RNA-seq), a next-generation sequencing (NGS) method largely used for the genome-wide measurement of RNA abundance and the detection of alternative splicing events [ 5, 6].
However, these methods require complex implementation and powerful calculators to reduce time calculation which makes simple methods, like worst month method, still largely used in sizing simple remote PV systems.
Furthermore, hand methods are largely used that are costly because they are time-consuming.
This method is largely used also to induce hypothermia in the fields [33].
The two-step method is largely used in the synthesis of nanofluids.
This method is largely used for investigation of anaerobic degradability of sludge and wastewater and toxicity of substrate.
The Delphi method is largely used in the healthcare setting as a reliable means of determining consensus for a defined clinical problem [28 30].
This method is largely used and recognized internationally.
This method is largely used also to induce hypothermia in the fields [ 33].
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