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The unique strength of SMRT technology relative to other next-gen sequencing methods is single-molecule reads that exceed 10 kb in length.
The top-5 methods are single resolution methods which generate one partition.
An additional method is Single Genome Amplification in which viral RNA is extracted from a sample and copied into cDNA, which in turn is subjected to limiting dilution and PCR amplification.
These methods are single-step and the update operation is only a single rotation.
Other methods are single-pass, storing summaries of past data as they are scanned [25].
Note that since all the methods are single-copy routing schemes, average path length corresponds to average transmission count.
The proposed multimode method is single-parameter, non-iterative and offers simplicity, automaticity and robustness.
Similar to the rotation assay, our method is single-cell based and permits the study of behaviors of individual cells as well as the average behavior in a population.
This method is single-point analysis.
An alternative method is single-molecule PCR (Kraytsberg & Khrapko, 2005).
The method is single-chip based, i.e. it separately uses the intensities for each selected chip.
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