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This region was amplified from tuber cDNA using specific oligonucleotides (see Methods), inserted, in antisense orientation, under the control of the tuber-specific patatin B33 promoter [ 3, 10] and introduced in potato (cv. Desirée) using Agrobacterium-mediated transformation [ 11].
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The whole DNA sequence was synthesized by a one-step total gene synthesis method, and then inserted in pUC18 vector.
The deletion cassettes were transformed in CEN.PK113-7D CEN.PK113-7Dum acetate method [ 75], inserted in the genome by homologous recombinathen and the selithium of tracetateants was done in YPD plates containing 200 μg/method8.
The overexpression cassettes were transformed in CEN.PK113-7D CEN.PK113-7Dum acetate method [ 75], inserted in the genome by homologous recombinathen and the selithium of tracetateants was done in YPD plates containing G418.
Methods: Button batteries were inserted in esophagus of 40 rabbits which were divided into four groups.
The proposed method inserted six edges that did not exist in the true pathway.
Information on foods, polyphenols, analytical methods and composition was manually inserted in the Microsoft Access® database.
In one method, parallel pin pairs are inserted in the distal and proximal bone segment using a drill guide.
In the first method, a specific preamble is inserted in which only a selected subset of subcarriers is used for transmission.
One of the main contributions of this work consists in proposing a method in which data is inserted in the LH2, HL2, and HH2 subbands after their decompositions into Karhunen Loeve components [ 22].
In the proposed method, this created watermark image is inserted in the original image.
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