When required, we used other methods, including real-time PCR, long PCR and array CGH.
Since all RNA quantitation methods, including real-time RT-PCR, have their limitations, further validation of the differential gene expression pattern might need to be carried out.
Most recently, culture-independent methods including real-time PCR and high-throughput sequencing were used to characterize the structure of fecal microbiota in cirrhotic patients.
Polymerase chain reaction (PCR) methods offer great potential in this respect and several methods, including real-time PCRs have been evaluated for bTB applications [ 18- 21].
Here, we studied the individual steps of NS5B-catalyzed RNA synthesis by a combination of biophysical methods, including real-time 1D H NMR spectroscopy.
To investigate the underlying molecular basis of the acquired resistant cells to paclitaxel in breast cancer, we used multiple methods including real-time RT PCR, western blotting analysis, migration and invasion assays, wound healing assay, and transfection.
Recently, Lourdault and his colleagues compared different routes (i.p., c.j. and s.c. inoculation) of infection and the dissemination of leptospries in blood and tissues of guinea pigs using multiple methods including real-time PCR [ 16].
Molecular methods including real-time polymerase chain reaction (PCR) and loop-isothermal amplification have been described, which can be applied to DNA extracted directly from the clinical sample, and these can provide a more rapid diagnosis but have a lower diagnostic sensitivity than culture.
Several methods can be used to quantify the abundance of specific AMF in roots, and these methods include real-time polymerase chain reaction (PCR) [53] [56].
The most commonly used methods include real-time PCR and microarray which can cause thermodynamic biases.
