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Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine.
Nevertheless, conventional methods in tissue engineering involving the seeding of cells into biodegradable scaffolds have intrinsic shortcomings, such as inflammatory reactions and fibrous tissue formation caused by scaffold degradation.
This study tested the cytotoxicity of a BDNF blended chitosan scaffold with human umbilical cord mesenchymal stem cells (hUC-MSCs), and the in vitro effect of BDNF blended chitosan scaffolds on neural stem cell differentiation with the aim of contributing alternative methods in tissue engineering for the treatment of traumatic brain injury (TBI).
J. L. West, "Modification of Materials With Bioactive Peptides", in Biopolymer Methods in Tissue Engineering, vol. 238, A. P. Hollander and Hatton, P. V. New Jersey: Humana Press, 2003, pp. 113 - 122.
By using the latest methods in tissue preservation and high-resolution electron tomography, Doroquez, Berciu et al. have, in effect, been able to transform old-fashioned black and white 'snapshots' into breathtaking 3D-models and movies.
Herpesvirus was determined by molecular methods in tissue samples from a male fin whale juvenile (Balaenoptera physalus) and a female common minke whale calf (Balaenoptera acutorostrata) stranded on the Mediterranean coast of the Region of Valencia (Spain).
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They tested the method in tissue isolated from the fish's noses, which contain magnetite.
Because of nature of the human body, the most used method in tissue three-dimensional data acquisition is magnetic resonance imaging (MRI) and computed tomography (CT) [ 28].
Several applications of fibers spun by both methods, especially in tissue engineering, organ function regeneration and drug delivery are introduced.
This chapter will present the most relevant methods explored in tissue engineering research for the production of three-dimensional, functional scaffolds for the regeneration of the dental tissues namely periodontal complex, endodontic tissues, and whole tooth.
We first validated the MiRABELLE method in tissues where the abundance of a particular miRNA had been experimentally increased.
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