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Finally, new methods in protein manipulation, including the site-specific incorporation of unnatural amino acids in vivo and the directed evolution of proteins, show promise in elucidating neural function with greater precision and flexibility.
The potential of esterases as enantioselective biocatalysts has enlarged in the last few years due to the progresses achieved in different areas, such as screening methodologies, overproduction of recombinant esterases, structural information useful for understanding the rational behind enantioselectivity, and efficient methods in protein engineering.
The general inability to unambiguously and consistently distinguish between the decoys and the native structure is one of the weaknesses of current theories and methods in protein folding, as well as in ab initio protein structure prediction [14].
Currently, heteronuclear 3D or higher-dimensional NMR experiments are standard methods in protein NMR spectroscopy [63], and one point of emphasis in this current work was to test the feasibility of using heteronuclear 3D NMR spectroscopy in NMR-based metabolome analysis.
Comparing with other several existing algorithms, the results have demonstrated the effectiveness of our proposed methods in protein function prediction.
As a result, the network based approach showed better performance than the raw scores of GC-based methods in protein function prediction [ 10].
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This gold standard positive set will increase the strength of machine learning methods in protein-protein interaction detection.
(http://www.biomedcentral.com/1752-0509/4/S1/S6). Huang [ 22] et. al. developed an algorithm for protein studies (http://www.biomedcentral.com/1471-2164/11/S3/S14). Wang et. al. [ 18] provided advances in clustering methods in proteins.
These results point to the prospect of using RCCL as a promising method in protein detection in the future.
Potential usefulness of this method in protein engineering and research are discussed.
Ion exchange chromatography (IEC) is a widely applied method in protein purification.
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