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For 22 of the subsets, the proportion of co-PPIs vis-à-vis the parent reference set was increased; the exceptions were multiple database evidence in fly, and for multiple experimental methods in mouse.
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Particularly, there is a lack of reliable models and testing methods in mice.
Thus, the goal of this study was to explore the antinociceptive potential, safety and mechanism of action of novel 1-pyrazole methyl ester derivatives, which were designed by molecular simplification, using in vivo and in vitro methods in mice.
The same research group [100] carried out a comparative research on the anti-inflammatory effect of aboveground and WERDN collected at the same time and in the same place by using the auricle swelling experiment, cotton ball granuloma and peritoneal fluid methods in mice.
DOI: http://dx.doi.org/10.7554/eLife.09275.010 To study whether the morphine-induced increase in presynaptic glutamate release in VTA-DA neurons was via presynaptic disinhibition, we observed the effect of 'closing' local GABAergic interneurons on the morphine-induced increase in glutamate release in VTA-DA neurons using optogenetic methods in mice.
Also, expression of SIRT1-FL and SIRT1-ΔExon8 mRNA levels was calculated relative to the housekeeper β-Actin using the delta C t) method in mouse tissues and cell lines.
By testing three M. tuberculosis H37Rv samples, one deletion mutant, and one recent clinical isolate for survival by the quantitative organ CFU counting method in mouse or guinea pig aerosol or intravenous infection models, we could discern no increase in bacterial fitness as a result of passaging of M. tuberculosis strains in mice prior to quantitative virulence testing in two animal models.
Since SirT1 overexpression has been reported to protect the murine heart from PQ-induced oxidative stress [ 16], we measured ROS content by dichlorofluorescein diacetate (CM-DCFDA) method in mouse cardiomyocytes to shed light on the impact of IGF-1/SirT1 signaling on oxidative stress generated by Ang II and by PQ.
They report this week in Nature Methods that in mouse embryos, the first differentiation of cell types happens when the embryo grows from eight to 16 cells.
Anticoagulant potential of test compounds was also assayed by in vivo tail BT method in mice [20].
Also, spillover from tissues close to the brain could introduce errors to the VOI-based analysis method in mice.
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