Sentence examples for methods in live from inspiring English sources

The detailed understanding of the functions and mechanisms of the actin and microtubuli cytoskeleton depended, besides innovative methods in live cell imaging, on the purification and labeling of its constituents.

Briefly, two lines of rainbow trout, a lean muscle line (L) and a fatty muscle line (F), were obtained after three generations of divergent selection for high or low muscle fat content, evaluated using a non-destructive method (Distell Fish Fatmeter) in live fish.

Noninvasive therapeutic cell tracking methods in living animals are important for understanding cell function and fate in connection with cell therapy.

The application of these methods in living cells is far from trivial, although recent work has achieved a remarkable progress in this direction [2] [7].

Therefore we think that SYFP2-mStrawberry, mKO-mCherry and mOrange-mCherry are the FRET pairs of choice for detecting protein-protein interactions by donor based quantitative FRET methods in living cells.

The next step was to test our method in live tissue preparations.

We tested the method in live preparations of pig left and right ventricular myocardium (thickness 8-18 mm) and phantoms imitating the optical properties of myocardial tissue.

This "in live cell" method may provide certain advantages over affinity purifications performed in cell extracts.

Therefore, we would encourage the authors to validate some of their measurements using different fixation methods, and in live cells if at all possible.

To this end, we measured FRET by the sensitized emission method in living cells.

Hence, the development of effective monitoring methods of O2− in live cells and in vivo is of great importance for ascertaining the onset and progress of related diseases.