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The analysis identified 250 Atf1 binding sites that located at the probable promoter regions (that were defined as the upstream intergenic sequence up to 1Kb from the start codon [41] or 150bps from the TSS [42] (see Materials and Methods) in cells after H2O2 treatment.
ZFN activity was validated by the presence of genome modifications with a mutation detection assay (see Materials and methods) in cells transfected with mRNA encoding for ZFN but not in cells transfected with a GFP-expressing plasmid as a negative control (supplementary material Fig. S1B).
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The numerical advantages of the diffusing-vortex scheme over other conventional vortex methods, Cloud-in-Cell methods, particle methods, and some finite difference schemes are evaluated in terms of reducing total CPU time, avoiding cutoff procedures, and sidestepping various interpolations.
When combined with new technical methods in cell processing and culturing, the therapeutic potential of T cells manufactured in academic centers has improved dramatically.
Some molecular approaches to cell biology using D. discoideum are detailed in Methods in Cell Biology.
Metabolic events were detected according to the protocols from Methods in Cell Biology.
To minimise delivery losses using the long cannula we tested several vector delivery methods in cell culture.
The procedure for how to purify these cells has been described in detail in several publications and summarized in Current Protocols in Cytometry [ 44] and Methods in Cell Biology [ 14].
After overnight growth, we detected SDS-stable Sup35 NM aggregates ('Materials and methods') only in cells producing the New1 fusion protein.
This paper introduces a technique for analyzing time integration methods used with the particle weight equations in δf method particle-in-cell (PIC) schemes.
The most extensively studied method in cell immobilization is entrapment.
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