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When combined with new technical methods in cell processing and culturing, the therapeutic potential of T cells manufactured in academic centers has improved dramatically.
Some molecular approaches to cell biology using D. discoideum are detailed in Methods in Cell Biology.
Metabolic events were detected according to the protocols from Methods in Cell Biology.
To minimise delivery losses using the long cannula we tested several vector delivery methods in cell culture.
The procedure for how to purify these cells has been described in detail in several publications and summarized in Current Protocols in Cytometry [ 44] and Methods in Cell Biology [ 14].
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The numerical advantages of the diffusing-vortex scheme over other conventional vortex methods, Cloud-in-Cell methods, particle methods, and some finite difference schemes are evaluated in terms of reducing total CPU time, avoiding cutoff procedures, and sidestepping various interpolations.
The analysis identified 250 Atf1 binding sites that located at the probable promoter regions (that were defined as the upstream intergenic sequence up to 1Kb from the start codon [41] or 150bps from the TSS [42] (see Materials and Methods) in cells after H2O2 treatment.
To better understand the contribution of different cell types in SLE pathogenesis, we applied the two methods in cell-type-specific differential expression analysis, csSAM and DSection, to identify cell-type-specific gene expression differences in heterogeneous gene expression measures obtained using RNA-seq technology.
The most extensively studied method in cell immobilization is entrapment.
Cellular membranes were lysed by a freeze thaw method in cell lysis solution (0.2% Triton X-100, 10 mM Tris (pH 7.0), and 1 mM EDTA).
The biotin avidin system quickly became a central method in cell biology, immunology, and protein engineering, as well as electron microscopy.
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