Such methods, however, suffer from limited resolution power, RNA transcript 3' heterogeneity, and time-consuming preparative purification steps.
EM-based methods, however, suffer from computational cost.
Existing methods, however, suffer from two main shortcomings.
These methods, however, suffer from low mass selectivity.
Most gene-randomizing methods, however, suffer from increased false-positives [ 26].
Both methods, however, suffer from limitations, including user-dependency and stringent technical requirements [ 4, 6- 12].
9– 16 All of these methods, however, suffer from one or more important limitations, including lack of air stability of the 2-pyridyl building blocks, 10, 12– 16 use of toxic metals, 9 inability to isolate the building blocks in chemically pure form, 11 and inefficient couplings with more challenging halide coupling partners such as deactivated aryl chlorides.
The first method, however, suffers from low yield and is expensive.
The MCMC method, however, suffers from intensive computation; therefore, it can be only applied to small datasets, for example, 20 30 individuals with 500 SNPs.
These methods, however, may suffer from low reproducibility and poor spatially controlled deposition.
