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Available methods like microarray techniques, real-time RT-PCR, and bead-based oligonucleotide methods have sensitivities in the subattomole range (Naciff et al., 2005a).
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These commonly used methods have sensitivity ranging from 11 to 85%% and specificity from 32to99%9 %.
Most methods have sensitivity above 90%, the minimum value recommended by the American Heart Association AHAA) for SAA on artifact-free ECG [ 18].
These methods have sensitivity comparable to those of microscopy and culture [ 15– 18], but are considerably less sensitive than Enzyme Linked Immunosorbent Assay (ELISA) or radio-immunoassay [ 19, 20].
The molecular methods had sensitivity of 50 UI/mL [ 19], and all serum samples were stored at −20°C until analysis.
The combined set of hits from both pipeline methods has sensitivity approaching that of unfiltered search; although there are some sequences which are not found by either method, they are few in number.
No single method has proved itself vastly superior to others [21] and different methods have different sensitivities to identical background conservation [22].
Different methods have different sensitivities on the category of protein targets.
Because the two methods have different sensitivities, the magnitude of the change determined by microarray and real time PCR is not the same.
Currently available serologic methods have variable sensitivities, specificities, and cross-reactivities, depending on the species being tested and the region where tests are performed.
To recognize positive candidates in a library, a decision method should have sensitivity and specificity as high as possible.
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