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Through this method, we have accomplished, for the first time, a rapid, semi-automated, and highly sensitive ChIP assay to investigate the genome-wide landscape of histone modification H3K4me3 using 1 000 mouse epiblast cells at E6.5, and found that the H3K4me3 landscape of post-implantation epiblast is more similar to that of the mEpiSCs than that of mESCs.
Direct sequencing methods, which represent >80% of overall costs, can be also replaced by other less expensive and more straightforward molecular methods [reviewed in 3] once researchers have accomplished a first preliminary survey using our general approach.
What would we have accomplished?
They have accomplished their mission".
Many have accomplished less".
"With that, you have accomplished something.
What you have accomplished is super-human.
"We think we have accomplished that objective".
What would higher taxes have accomplished?
"I will have accomplished something".
Trigon seems to have accomplished that feat.
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