Sentence examples for methods generated short from inspiring English sources

Both methods generated short reads that were then realigned to a reference sequence.

The presence of repeats is still a major problem in shotgun sequencing, and with a possible shift from Sanger sequencing towards high throughput methods generating short reads without mate pairs, the repeat problem will continue to cause errors in shotgun assemblies.

A simple mapping method that maps the generated short read data onto a published reference genome cannot cover the species-specific regions divergent from a reference genome.

A striking advantage of using the Roche/454 sequencing method compared to other second-generation DNA sequencing methods that generate shorter reads is the potential to identify insertions in the target genome with breakpoints defined at the base pair resolution, as demonstrated in the Watson genome analysis [ 56].

Here we tested Illumina metabarcoding, a method that generates shorter reads but achieves deeper sequencing than 454 metabarcoding approaches.

Two different methods have been used to generate short hairpin RNA (shRNA) libraries; one is to use chemically synthesized oligonucleotides, and the other is to convert complementary DNAs (cDNAs) into shRNA cassettes enzymatically.

This method employs two restriction enzymes to generate short fragments in the analysis of AFLP.

These methods run millions of sequencing reactions in parallel, generating short sequence reads on the gigabase-scale, and harvest assembled contigs by algorithms.

Although this method generates very short sequence reads, several recent studies have demonstrated that it is possible to assemble these short reads into good quality draft genome sequences [ 30- 41].

Irrespective of the best protocol used for generating short sequence tags, our study provides insight into the potential of short tag sequencing as a method for investigating and inferring differential expression when different reference transcriptomes are available.

Dicer generates short RNA sequences of about 22-nucleotides.