However, these methods generally require large equipment such as a pumper and vacuum car, and other special devices.
On the other hand, the conventional macro-level methods generally require one or more interaction parameters in order to capture the interaction of stress components in the matrix and fibres, while MMF uses a micromechanical model to account for the stress interaction, so that the interaction parameter is not needed (Xu et al. 2015a, b).
Unfortunately, these methods generally require nonphysiological fixation of cells.
However, these experimental methods generally require large amounts of resources and are very time consuming.
Network alignment methods generally require a search algorithm to detect high-scoring subgraphs from a single or several weighted graphs.
However, these methods generally require manual input, thereby limiting the throughput of the experiment and increasing the susceptibility to cross-contamination and nonspecific amplification.
Nevertheless, qualitative methods generally require special training and tend to be time-consuming for participants, researchers, and those who must analyze the resulting data.
Numerous proteins can be converted to amyloid-like fibrils to increase cytotoxicity and induce apoptosis, but the methods generally require a high concentration of protein, vigorous shaking, or fibril seed.
However, these methods generally require a high concentration of protein, vigorous shaking, or the addition of fibril seed and are not easy to isolate fibrillar protein from others such as oligomers, protofibrils or non-fibrillar proteins that may also exist in the solution [ 1, 2, 7, 15, 16, 18, 19].
The chemical method generally requires the services of specific reaction conditions such as required pH value and temperature.
