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We describe methods for whole cell ultrastructural imaging that can provide three-dimensional views of intraerythrocytic development.
In methodological terms, the work presented here underscores the role and importance of reliable optimisation methods for whole technology networks.
This procedure is outlined in Fig. 1, where markers for each cell are detected from fluorescence, or marked by hand, with two alternative methods for whole cell detection: fluorescence and the projections.
The aim of this study was to design a set of new potential blood-detectable biomarkers for prenatal DS screening by computational data mining, that is more focused on DS screening than currently available commercial multiplex kits or high-throughput methods for whole proteome analysis.
Microwave-assisted acid digestion was conducted using previously described methods for whole blood [ 24, 26].
Alternative methods for whole mount preparations featuring different staining reagents and preparations have been reported that preserve antigenicity so that tissue can later be sectioned and immunostained [ 26].
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The main focus of our current work is on advancing theoretical and experimental methods for whole-body time resolved imaging and fluorescence lifetime imaging.
He held a postdoctoral appointment at Stanford University where he developed high-throughput microoptofluidic methods for whole-genome amplification of DNA from individual, uncultivated microbial cells in Prof. Stephen Quake's laboratory.
He writes that he feared that if the majority opinion stands, it "will likely have broad consequences, such as preempting methods for whole-genome sequencing".
Standard methods for whole-mount in situ hybridisation were used to detect mRNA expression.
Many methods for whole-genome analysis are too computationally intensive for standard desktop computers.
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