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However, methods for serotyping directly on the clinical specimen remain scarce.
Our results suggest that these RT-PCR assays are useful alternatives to existing methods for serotyping DENV in clinical sera.
This indicates that the DENV1, DENV2, DENV3 and DENV4 assays are useful alternatives to other methods for serotyping DENV.
EIP may also have a role in the development and validation of culture-independent methods for serotyping, subtyping, virulence profiling, and antimicrobial drug resistance testing.
The theoretical and clinical validation of these DENV serotype-specific RT-PCR assays suggest that they are good alternatives to existing methods for serotyping DENV in clinical samples.
It is likely that many isolate-based methods for serotyping, pulsed-field gel electrophoresis, and antimicrobial drug testing will need to transition to sequence-based techniques to remain epidemiologically useful.
Similar(54)
Lastly, we used a single-colony method for serotyping in both studies.
In the future, it will therefore be possible to test also serotype 6C in accordance to the WHO recommended standard method for serotyping of S. pneumoniae.
In future, it will, therefore, be possible to test also serotype 6C in accordance to the standard method for serotyping of S. pneumoniae recommended by WHO.
HEVs have long been classified on the basis of serotype-specific antisera in virus neutralization tests (1, 10 ), the only method available for serotyping HEVs.
Many of the studies, including most of the strains from the S. suis MLST Database, do not specify the method used for serotyping.
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