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He pioneered methods for sequencing the first bacterium, Haemophilus influenzae, and raced the government to a draw in sequencing a draft version of the human genome in June 2000.
Previous methods for sequencing native RNA strands involved degrading one chemical base, or letter, at a time, and these techniques have changed little since their invention in the late 1970s2.
Such an approach was out of the question until the development a few years ago of methods for sequencing very large amounts of DNA at low cost.
For that, the samples were sent to Beth Shapiro, author of How to Clone a Mammoth and an expert on ancient DNA, whose paleogenomics lab at the University of California, Santa Cruz, excavates animal bones in the Artic permafrost and has perfected methods for sequencing old DNA.
Some of the world's leading companies, including Google, Qualcomm and Nokia, have sponsored big-money contests challenging competitors around the world to design a host of wonders, including robots that can explore the moon, superefficient electric vehicles and more accurate methods for sequencing the human genome.
To conclude, we discuss the development of newborn screening assays in dried blood spots and high-throughput methods for sequencing the protein-coding regions of the genome (whole exome sequencing) and their relevance to future changes in the MPS III diagnostic landscape.
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Experts are likely to debate which team's method for sequencing the human genome is better.
Celera had a top-down method for sequencing the genome, the public consortium a bottom-up approach.
Dr. Wu developed the first method for sequencing DNA and some of the fundamental tools for DNA cloning in 1970.
Now, sequencing technology permits transcriptome analysis of single neurons31,32, and we recently developed a method for sequencing single nuclei that preserves activity-dependent gene expression33.
British biochemist Fred Sanger develops the "dioxy method" for sequencing DNA, using it to sequence the first DNA genome – that of a virus.
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